Comparative chemotaxonomic studies of mycolic acid-free coryneform bacteria of human origin.

摘要: Abstract Forty-two clinical isolates were classified as Corynebacterium minutissimum, striatum, and CDC group I by the API Coryne system. The chemotaxonomic characteristics of determined thin-layer chromatographic analysis. Twenty-six found to have a type IV cell wall (meso-di-aminopimelic acid arabinose, galactose) but did not contain mycolic acids. These 26 shared with those acid-free reference strains (including amycolatum NCFB 2768 strain, "Corynebacterium asperum," coryneform groups I2 F2). total protein profiles sodium dodecyl sulfate-polyacrylamide gel electrophoresis similar each other that C. strain. asperum" (CIP 100836, CIP 80.54, 79.37, 52.13), bacteria F2 (CDC F5771, F5890, G723, G1970), closely related. Thus, acid-negative chemotype may belong single taxon. DNA hybridization studies could confirm this hypothesis. present study shows importance analysis for verifying strain identifications completing results from biochemical tests, particularly bacteria.