SDS-PAGE for identification of species belonging to the Mycobacterium fortuitum complex

摘要: We performed a study to determine the usefulness of SDS-PAGE whole cell proteins for characterization species rapidly growing mycobacteria belonging Mycobacterium fortuitum complex. Strains included 37 M. fortuitum, 32 chelonae, 10 peregrinum, 5 abscessus, and 3 mucogenicum. Eight collection strains (including type five species) were also in study. All yielded between 44 58 bands electrophoretograms. Intraspecies similarity showed Dice coefficients higher than 95%, with only one strain having six-band difference (Dice coefficient 87.75%). However, interspecies was always below 75%, being peregrinum (75.51%) chelonae abscessus (54.9%). Visual examination electrophoretograms sufficient characterization. is useful technique identification isolates complex, easy perform without need complex or expensive equipment.