Regulation of human lymphocyte proliferation by a tumour cell-derived DNA fraction

摘要: A suppressor activity was isolated and partially purified from culture supernatant of human myeloid leukaemic cell line HL-60 by ion-exchange chromatography, HPLC sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This capable suppressing proliferation mitogen-stimulated normal lymphocytes in a dose-dependent manner. The identified as DNA its typical absorbance at 260 nm, susceptibility to DNase digestion. with suppressive found be double-stranded the molecular weight range between 0.6 1.5 kb. material did not have cytotoxic alter interleukin-2 receptor expression. Cell cycle analysis has suggested that molecules could interact arrest stimulated G1 phase, apparently preventing cells entering into proliferating S phase.