Structure and secretion of CofJ, a putative colonization factor of enterotoxigenic Escherichia coli
作者： Alex S. W. Yuen , Subramaniapillai Kolappan , Dixon Ng , Lisa Craig
DOI: 10.1111/MMI.12407
关键词:
摘要: Enterotoxigenic Escherichia coli (ETEC) colonize the human gut, causing severe cholera-like diarrhoea. ETEC utilize a diverse array of pili and fimbriae for host colonization, including Type IVb pilus CFA/III. The CFA/III machinery is encoded on cof operon, which similar in gene sequence synteny to tcp operon that encodes another pilus, Vibrio cholerae toxin co-regulated (TCP). Both operons possess syntenic encoding protein unknown function. In V. cholerae, this protein, TcpF, critical colonization factor secreted by TCP apparatus. Here we show corresponding CofJ, soluble via We present 2.6 A resolution crystal structure revealing large β-sandwich bears no or structural homology TcpF. CofJ has cluster exposed hydrophobic side-chains at one end pore-forming proteins perfringolysin O α-haemolysin. binds lipid vesicles epithelial cells, suggesting role membrane attachment during colonization.
rcsb.org参考文章(89)
Kara S. Giddings, Arthur E. Johnson, Rodney K. Tweten, CHAPTER 38 – Perfringolysin O and intermedilysin: mechanisms of pore formation by the cholesterol-dependent cytolysins The Comprehensive Sourcebook of Bacterial Protein Toxins (Third Edition). pp. 671- 679 ,(2006) , 10.1016/B978-012088445-2/50043-3
Rolf Köhler, Karsten Schäfer, Shirley Müller, Guillaume Vignon, Kay Diederichs, Ansgar Philippsen, Philippe Ringler, Anthony P. Pugsley, Andreas Engel, Wolfram Welte, Structure and assembly of the pseudopilin PulG Molecular Microbiology. ,vol. 54, pp. 647- 664 ,(2004) , 10.1111/J.1365-2958.2004.04307.X
Anastassis Perrakis, Richard Morris, Victor S. Lamzin, Automated protein model building combined with iterative structure refinement. Nature Structural & Molecular Biology. ,vol. 6, pp. 458- 463 ,(1999) , 10.1038/8263
David A Cisneros, Peter J Bond, Anthony P Pugsley, Manuel Campos, Olivera Francetic, Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation The EMBO Journal. ,vol. 31, pp. 1041- 1053 ,(2012) , 10.1038/EMBOJ.2011.454
T Taniguchi, Y Fujino, K Yamamoto, T Miwatani, T Honda, Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili. Infection and Immunity. ,vol. 63, pp. 724- 728 ,(1995) , 10.1128/IAI.63.2.724-728.1995
Thomas J. Kirn, Niranjan Bose, Ronald K. Taylor, Secretion of a soluble colonization factor by the TCP type 4 pilus biogenesis pathway in Vibrio cholerae Molecular Microbiology. ,vol. 49, pp. 81- 92 ,(2003) , 10.1046/J.1365-2958.2003.03546.X
Tiina Palomäki, Richard Pickersgill, Ruusu Riekki, Martin Romantschuk, Hannu T. Saarilahti, A putative three-dimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in Erwinia carotovora. Molecular Microbiology. ,vol. 43, pp. 585- 596 ,(2002) , 10.1046/J.1365-2958.2002.02793.X
S Knutton, M M McConnell, B Rowe, A S McNeish, Adhesion and ultrastructural properties of human enterotoxigenic Escherichia coli producing colonization factor antigens III and IV. Infection and Immunity. ,vol. 57, pp. 3364- 3371 ,(1989) , 10.1128/IAI.57.11.3364-3371.1989
A P Pugsley, The complete general secretory pathway in gram-negative bacteria Microbiological Research. ,vol. 57, pp. 50- 108 ,(1993) , 10.1128/MMBR.57.1.50-108.1993
Thomas C. Terwilliger, Joel Berendzen, Automated MAD and MIR structure solution Acta Crystallographica Section D-biological Crystallography. ,vol. 55, pp. 849- 861 ,(1999) , 10.1107/S0907444999000839