Role of tetanus neurotoxin insensitive vesicle-associated membrane protein in membrane domains transport and homeostasis.

摘要: Biological membranes in eukaryotes contain a large variety of proteins and lipids often distributed domains plasma membrane endomembranes. Molecular mechanisms responsible for the transport organization these along secretory pathway still remain elusive. Here we show that vesicular SNARE TI-VAMP/VAMP7 plays major role composition transport. We found exogenous endogenous GPI-anchored was altered fibroblasts isolated from VAMP7-knockout mice. Furthermore, disassembly reformation Golgi apparatus induced by Brefeldin A treatment washout were impaired VAMP7-depleted cells, suggesting loss VAMP7 expression alters biochemical properties dynamics apparatus. In addition, lipid profiles knockout cells indicated defect glycosphingolipids homeostasis. conclude is required effective GPI–anchored to cell surface t...