Communication of the Position of Exon-Exon Junctions to the mRNA Surveillance Machinery by the Protein RNPS1

摘要: In mammalian cells, splice junctions play a dual role in mRNA quality control: They mediate selective nuclear export of mature and they serve as mark for surveillance, which subjects aberrant mRNAs with premature termination codons to nonsense-mediated decay (NMD). Here, we demonstrate that the protein RNPS1, component postsplicing complex is deposited 5' exon-exon junctions, interacts evolutionarily conserved human Upf complex, central NMD. Significantly, RNPS1 triggers NMD when tethered 3' untranslated region beta-globin mRNA, demonstrating its subunit directly involved surveillance.