F-actin distribution in the ovaries of pre-vitellogenic and vitellogenic black blowflies, Phormia regina (Meigen) (Diptera : Calliphoridae)

摘要: Abstract The spatial distribution of F-actin microfilaments in the ovaries previtellogenic and vitellogenic female black blowflies, Phormia regina (Diptera : Calliphoridae), as females shift from a sugar to liver diet, is determined using rhodamine-labelled phalloidin (rh-phalloidin). During pre-vitellogenic stages ovarian development (i.e. corresponding diet) single bright fluorescent layer marks interface between follicle cells oocyte. Fluorescence also most evident at inner surface ring canals nurse cells. This observed both distal part germarium, growing However, when liver-fed necessary for vitellogenesis), 2 layers are cell-oocyte interface. In addition, cytoplasm during vitellogenesis appears full actin rings found increase size thickness. changing organization follicles process egg chamber oocyte formation discussed possible functions considered.