10 – Expression of Human Neuropeptide Y Gene in Neuronal Cell Lines

摘要: Publisher Summary This chapter discusses the expression of human neuropeptide Y (NPY) gene in neuronal cell lines. To define sequences that are important for transcription NPY gene, deletion constructs containing various amounts 5'-flanking DNA generated and tested their ability to support a heterologous reporter vivo transient transfection assays. In vitro analyses performed verify initiating from authentic start site. identify elements involved binding regulatory proteins, extracts prepared lines expressing presence factors able form specific complexes with regions shown be regulating transcriptional activity this gene. These types experiments should determine which different cells whether DNA-binding activities involved.