A high-efficiency HeLa cell nuclear transcription extract.
作者： DAVID J. SHAPIRO , PHILLIP A. SHARP , WALTER W. WAHLI , MARTHA J. KELLER
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摘要: A HeLa cell nuclear transcription extract that is approximately 20 times more efficient than standard extracts was developed. Transcription of the strong adenovirus II major late promoter by this results in synthesis 1.5-4 molecules product RNA per molecule template, indicating capable multiple rounds initiation. Standard transcribe closed circular and linear templates with equal efficiency. In contrast, new exhibits an increase twofold on a circular, as opposed to linear, template.
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