Characterization and Comparative Profiling of MicroRNAs in a Sexual Dimorphism Insect, Eupolyphaga sinensis Walker

摘要: Background MicroRNAs are now recognized as key post-transcriptional regulators in animal ontogenesis and phenotypic diversity. Eupolyphaga sinensis Walker (Blattaria) is a sexually dimorphic insect, which also an important source of material used traditional Chinese medicine. The male E. have shorter lifecycles go through fewer instars than the female. Furthermore, males forewings, while females totally wingless. Results We Illumina/Solexa deep sequencing technology to sequence small RNA libraries prepared from fourth-instar larvae female sinensis. 19,097,799 raw reads were yielded total: 7,817,445 library 11,280,354 male, respectively. As result, we identified 168 known miRNAs belonging 55 families well 204 novel miRNAs. Moreover, 45 showed significantly different expression between larvae, validated 10 them by Stem-loop qRT-PCR. Some these differentially expressed related metamorphosis, development diversity. Conclusions/Significance This first comprehensive description results provide useful resource for further in-depth study on molecular regulation evolution These findings not only enrich hemimetabolans but lay foundation phenomena sexual dimorphism.