Pro-angiogenic actions of Salvianolic acids on in vitro cultured endothelial progenitor cells and chick embryo chorioallantoic membrane model.

摘要: Abstract Aim of the study Salvia miltiorrhiza (SM, also known as DanShen) is one well-known widely-used Chinese herbal medicines in clinical practice. In this we aimed to demonstrate pro-angiogenic effects Salvianolic acids (SAs) treat illnesses such ischemic cardiovascular diseases, main active components aqueous extract SM. Materials and methods To do this, new-born rat spleen mononuclear cells were isolated endothelial progenitor (EPCs) expanded (not more than 24 h) SD. Then activities SAs evaluated on vitro cultured EPCs chick embryo chorioallantoic membrane (CAM) model. And adherent stained with DiI complexed acetylated low-density lipoprotein (DiI-acLDL) fluorescein Ulex Europaeus agglutinin-1 (FITC-UEA-1), then viewed by laser scanning confocal microscope (LSCM) confirm lineage. identification was tested ultrastructural analyses. proliferation, migration vasculogenesis activity assayed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, transwell chamber assay kit, respectively. adhesion performed replating those fibronectin-coated dishes, counting cells. Results phenotype confirmed presence double positive for DiI-acLDL uptake lectin binding Weibel–Palade body cytoplasm Incubation increased number promoted migratory, adhesive capacity. angiogenesis evidenced CAM Conclusions The results present suggest that may have utility therapeutic postnatal tissue, contributing benefit SM therapy patients coronary artery disease.