Positron emission tomography imaging for herpes virus infection: Implications for oncolytic viral treatments of cancer

摘要: Molecular therapy using viruses would benefit greatly from a non-invasive modality for assessing dissemination of viruses. Here we investigated whether positron emission tomography (PET) scanning [124I]-5-iodo-2′-fluoro-1-β-d-arabinofuranosyl-uracil (FIAU) could image cells infected with herpes simplex (HSV). Using replication-competent HSV-1 oncolytic thymidine kinase (TK) under control different promoters, demonstrate that viral infection, proliferation and promoter characteristics all interact to influence FIAU accumulation imaging. In vivo, as few 1 × 107 particles injected into 0.5-cm human colorectal tumor can be detected by [124I]FIAU PET signal intensity is significantly greater at 48 hours compared 8 after injection, demonstrating detect changes in TK activity resulting local proliferation. We also show the ability FIAU-PET differences infectivity 0.5 log increments. Non-invasive imaging might useful biologically relevant distribution virus therapies HSV.