Modulation of the estradiol-17 beta mitogenic effect on human breast cancer MCF-7 cells by serum albumin in defined medium.

摘要: The influence of bovine serum albumin on estradiol-17 beta growth and responsiveness human mammary cancer MCF-7 cells was studied compared to that dextran-coated charcoal-treated fetal calf other proteins. To reveal the protein factor role, a culture defined medium used. It composed minimal essential without phenol red, added with iron saturated tranferrin (10 mg/ml) porcine insulin (5 mg/ml). An mitogenic effect noticed only in present or serum. A concentration increase provokes greater submitted an estrogenic treatment. obtained results suggest participates mode action target modulates its effect. conditions appear for study estrogens antiestrogens estrogen-dependent cells.