Tamoxifen-induced Apoptosis in Breast Cancer Cells Relates to Down-Regulation of bcl-2, but not bax and bcl-XL, without Alteration of p53 Protein Levels
作者： Izo Kimijima , Seiichi Takenoshita , Atsuo Tsuchiya , Takanori Watanabe , Masahiko Kanno
DOI:
关键词: Downregulation and upregulation 、 Gene expression 、 Tamoxifen 、 Apoptosis 、 Cancer research 、 Gene product 、 Tumor suppressor gene 、 Biology 、 Bcl-xL 、 Estrogen
摘要: Tamoxifen (TAM) has been shown to induce apoptosis in breast cancer cells. bcl-2 family genes, which can interact with each other, have interfere after various stimuli. In this study, we investigated the effects of TAM on gene products bcl-2, bax, and bcl-XL p53 levels estrogen receptor-positive MCF-7 We found that induced time- concentration-dependent down-regulation at both mRNA protein level. Down-regulation correlated TAM-induced apoptosis. addition, estradiol treatment significantly increased expression blocked reduction by TAM. did not, however, affect bcl-XL, or Our results demonstrate a dose-dependent manner modulating cells, was not accompanied alterations levels.
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