Glucocorticoid receptor deficient thymic and peripheral T cells develop normally in adult mice.

摘要: The involvement of glucocorticoid receptor (GR) signaling in T cell development is highly controversial, with several studies for and against. We have previously demonstrated that GR - / mice, which usually die at birthbecause impaired lung development, exhibit normal least embryonic mice fetal thymus organ cultures. To directly investigate the role adult we analyzed few occasionally survive birth, irradiated reconstituted liver precursors. All thymic peripheral cells, as well other leukocyte lineages, developed were maintained levels. Anti-CD3-induced death thymocytes vitro, repertoire heterogeneity proliferation response to anti-CD3 stimulation absence signaling. Finally, show metyrapone, an inhibitor synthesis (commonly used demonstrate a glucocorticoids development), thymocyte regardless genotype indicating this reagent inhibits glucocorticoid-independent fashion. These data not required either or maintenance mice.