“Omics”-Enabled Microbial Sensors on Ocean Platforms
作者： Julie C. Robidart , Irina N. Shilova , Jonathan P. Zehr
DOI: 10.1007/978-1-61779-915-0_1
关键词:
摘要: In order to assess the diversity and function of microbial communities most effectively, molecular assays need be designed that target phylogenetic markers functional genes are key major ecological processes microorganisms. A streamlined design process is presented here designs probes for microarray quantitative PCR (qPCR) assays, application on lab bench or remote instrumentation. These can used DNA (genome) RNA (gene transcription) studies. The pipeline described establishes a database environmental sequences, which then microarrays inform qPCR detailed along with assay optimization process. Finally, high-density described. protocol currently Environmental Sample Processor, deployable robotic “genosensor” (http://www.mbari.org/esp). protocols should advance applications in oceanography using instrumentation as well traditional sampling methods.
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