Determination of total and pancreatic α-amylase in human serum with 2-chloro-4-nitrophenyl-α-d-maltotrioside as substrate

摘要: Abstract A reagent and assay conditions for the determination of catalytic concentration α-amylase (E.C. 3.2.1.1) in serum with 2-chloro-4-nitrophenyl-α- d -maltotrioside as substrate are described. The selected reaction mixture contains 50 mmol/l 2-(N-morpholino)ethanesulfonic acid buffer at pH 6.1 (37°C), 300 sodium chloride, 5 calcium chloride 450 potassium thiocyanate. described method is suitable measurement total well pancreatic by including antibodies against salivary isoenzyme. shows absence a lag phase period, sensitive precise, has large analytical range free from interference hemoglobin, bilirubin triglycerides. Comparative studies showed good correlation other established methods.