Involvement of Protein Kinase A in Patterning of the Mouse Somatosensory Cortex

摘要: Patterning of the mouse somatosensory cortex is unusually evident because presence a “barrel field.” Presynaptic serotonin and postsynaptic glutamate receptors regulate barrel formation, but little known intracellular signaling pathways through which they act. To determine whether protein kinase A (PKA) plays role in development field, we examined five viable PKA subunit-specific knock-out (KO) lines for field abnormalities. Barrels are present these mice, those lacking RIIβ subunit display significantly reduced contrast between cell densities hollows sides compared with wild-type animals. Thalamocortical afferent segregation posterior medial subfield appeared normal, suggesting site gene action protein. Immunoelectron microscopy confirmed that was selectively localized to dendrites dendritic spines. Mice show receptor (GluRA) insertion into density postnatal day 7 cortex; however, GluRA KO mice developed normal barrels. Our results clearly demonstrate differentiation. They also clear dissociation regulation trafficking by its formation. Finally, although downstream cAMP cannot be ruled out, data suggest may not principle target none mutants showed barrelless phenotype similar observed adenylate cyclase type 1 mice. These give insight activity-dependent mechanisms