In Situ Microspectrophotometric Studies on the Pigments of Single Retinal Rods

摘要: Three spectral entities have been observed in single intact frog rod outer segments at 506 mmu, 480 mmu and 380 mmu. It is likely that the peak of was somewhat altered by bleaching reactions originated about 510 This identified with 502 rhodopsin digitonin extracts. Spectra polarized light same maximum, identifying dichroism rods rhodopsin. The dichroic ratio around 6, giving segment an axial density 0.09/5mu or 0.9 OD total, a pigment concentration 2 to 3 mM. data are used compute angle separating molecular absorption vectors from perfect restriction plane. 16 degrees 23 depending on which two assumptions one chooses for type ordering. peaks thought correspond respectively metarhodopsin retinene. Disappearance former accompanied accumulation latter. reaction seems occur more slowly than corresponding solution. Spread spectra illuminated dark areas did not over distances mu greater. were similar point showing spectrally homogeneous both individually collectively.