Auxin and ETTIN in Arabidopsis gynoecium morphogenesis.

摘要: The phytohormone auxin has wide-ranging effects on growth and development. Genetic physiological approaches implicate flux in determination of floral organ number patterning. This study uses a novel technique transiently applying polar transport inhibitor, N-1-naphthylphthalamic acid (NPA), to developing Arabidopsis flowers further characterize the role organogenesis. NPA marked as well regional specification wild-type gynoecia, defined by morphological histological landmarks for boundaries, tissue-specific reporter lines. NPA's gynoecium patterning mimic phenotype mutations ETTIN, member response factor family transcription factors. In addition, application different concentrations reveal an increased sensitivity weak ettin alleles disruptions transport. contrast, defects found spatula gynoecia are partially rescued treatment with NPA. A model is proposed suggesting apical-basal gradient during provides mechanism linking ETTIN's putative transcriptional regulation auxin-responsive genes establishment or elaboration tissue gynoecial