Mitochondrial DNA variation of the Sumatran elephant in Sumatera

摘要: A research on Mitochondrial DNA analysis of genetic diversity in Sumatran elephant (Elephas maximus sumatranus) was conducted this study.  630 bp segment mitochondrial amplified 105 samples from 5 locations Sumatera (Bentayan, Sugihan, Bukit Salero Lahat, Seblat, Way Kambas) using a set primers: MDL3 (5’-CCCACAAT-TAATGGGCCC-GGAGCG-3’) and MDL5 (5’-TTACATGAATTGGCAGCCA-ACCAG-3’). The objectives study is to generate D-loop sequences for all the under and  provide information haplotypes nucleotide of  populations.             total PCR product were successfully sequenced perfectly, with an average length about 616 base pairs. However, fragments  used first 601 bases. Results showed six (BP, BT, BS, BR, BX BY) identified Sumatera.  most sampled individuals are haplotipe BT. BY likely new haplotypes..  All haplotype, except BP  belonging clade. haplotipe BX derived haplotype  was derived BS by one transversion respectively. other substitutions network transitions. haplotype BP widely distribute Sri Lanka, Sumatera, Peninsular Malay  China). Although reported that BU distributed Malay, but not detected study. Genetic distances within populations Bentayan, Sugihan Kambas ranged 0.0000 - 0003, distance between 0. 0022. elephant’s population low.• island low, highest is  region Buki Salero  Lahat and, lowest Bentayan Sugihan. Overall, results Fu Li's F * test statistic indicates elephants Sumatra -0.78871, which means there no inbreeding, significant at P> 0:10.   Keywords : elephant, Elephas maximus  sumatranus, DNA,