Acanthamoeba from human nasal mucosa infected with an obligate intracellular parasite

摘要: Summary An aggregation of numerous round particles could be observed inside the cytoplasm trophozoites an Acanthamoeba strain (Bn 9 ) isolated from human nasal mucosa [8]. Transmission and scanning electron microscopy revealed small coccoidal bacteria (0.4–0.6 μm in diameter) with a prominent trilamellar cell wall. Initially multiplied food vacuoles infected trophozoites, later on they also free cytoplasm. The affected host cells were finally ruptured liberating into environment. procaryotes ingested by further that not able to digest them but became lethally infected, too. parasitic successfully transfered pathogenic A. castellanii (C 3 ). Infected amoebae cured parasites rifampicin. taxonomic position gram-positive cocci determined. So far it was possible subcultivate organisms vitro. Therefore we assume are obligate intracellular amoebae, comparable those rod-shaped described Drozanski [3] as pathogens Acanthamoebae.