Chromatin remodeling in the aging genome of Drosophila.

摘要: Chromatin structure affects the accessibility of DNA to transcription, repair, and replication. Changes in chromatin occur during development, but less is known about changes aging. We examined state its effect on gene expression aging Drosophila at whole genome cellular level using whole-genome tiling microarrays activation repressive marks, transcriptional single-cell immunohistochemistry. found dramatic reorganization chromosomal regions with age. Mapping H3K9me3 HP1 signals fly chromosomes reveals young flies expected high enrichment pericentric regions, 4th chromosome, islands facultative heterochromatin dispersed throughout genome. With age, there a striking reduction this resulting nearly equivalent heterochromatin, euchromatin. These extensive marks are associated alterations age-related expression. Large-scale age further substantiated by immunohistochemistry that shows nuclear distribution Such epigenetic directly or indirectly impinge upon important functions such as expression, The combination genome-wide approaches immunoprecipitation studies conjunction shown here provide first step toward defining how may contribute process metazoans.