Long noncoding RNA LINC00515 promotes cell proliferation and inhibits apoptosis by sponging miR-16 and activating PRMT5 expression in human glioma

摘要: Introduction: In recent years, an increasing amount of literature has demonstrated the functional role long non-coding RNA (lncRNA) in human diseases. LINC00515 is a newly defined lncRNA and reported to act as oncogene multiple myeloma. However, function glioma still uncertain. Materials methods: We examined expression levels tissues cell lines using real-time PCR analysis. addition, we confirmed distribution cells suppressed with siRNAs. CCK-8, colony formation assay apoptosis analysis were used study progression. Then, bioinformatics prediction subsequent experiments reveal underlying molecular mechanism. Results: found that was up-regulated lines. mainly located cytoplasm cells. Knockdown led decreased proliferation increased Mechanistically, our data indicated there LINC00515/miR-16/PRMT5 regulatory axis glioma. could activate PRMT5 promote progression by acting sponge miR-16. Conclusion: elevated promotes growth inhibits The cascade plays critical