Acute heat shock leads to cortical domain internalization and polarity loss in the C. elegans embryo.

摘要: Many developmental processes are inherently robust due to network organization of the participating factors and functional redundancy. The heterogeneity involved their connectivity puts these at risk abrupt system collapse under stress. polarization one-cell C. elegans embryo constitutes such an process with However, how is affected by acute stress has not been thoroughly investigated. Here, we report that heat shock (34°C, 1 h) triggers a highly reproducible loss anterior posterior polarity domains. Temperature-dependent cortical non-muscle myosin II drastically reduces tension leads internalization large plasma membrane domains including membrane-associated factor PAR-2. After internalization, vesicles associated cluster around centrosomes thereby withdrawn from process. Transient formation domain suggests microtubule-induced self-organization this compromised after shock. Hence, our data uncover undergoes temperature-dependent