Mesenchymal stem cell proliferation and differentiation on an injectable calcium phosphate-chitosan composite scaffold.

摘要: Calcium phosphate cement (CPC) can be molded or injected to form a scaffold in situ, has excellent osteoconductivity, and resorbed replaced by new bone. However, its low strength limits CPC non-stress-bearing repairs. Chitosan could used reinforce CPC, but mesenchymal stem cell (MSC) interactions with CPC-chitosan have not been examined. The objective of this study was investigate MSC proliferation osteogenic differentiation on high-strength scaffold. MSCs were harvested from rat bone marrow. At powder/liquid (P/L) mass ratio 2, flexural (mean+/-sd; n=5) (10.0+/-1.1) MPa for CPC-chitosan, higher than (3.7+/-0.6) (p<0.05). P/L 3, (15.7+/-1.7)MPa (10.2+/-1.8)MPa Percentage live attaching scaffolds increased 85% at 1 day 99% 14 days. There (180+/-37) cells/mm(2) day; cells proliferated (1808+/-317) SEM showed healthy spreading anchored nano-apatite crystals via cytoplasmic processes. Alkaline phosphatase activity (ALP) (557+/-171) (pNPP mM/min)/(microg DNA) (159+/-47) Both (35+/-32) baseline ALP undifferentiated tissue-culture plastic In summary, had CPC. matched that the FDA-approved control. differentiated down lineage expressed high levels marker ALP. Hence, stronger may useful cell-based regeneration moderate load-bearing maxillofacial orthopedic applications.