Differential expression of S19 ribosomal protein, laminin-binding protein, and human lymphocyte antigen class I messenger RNAs associated with colon carcinoma progression and differentiation.

摘要: Three complementary DNA encoding S19 ribosomal protein (S19), laminin-binding (LBP), and HLA class I (HLA-I) genes were isolated from a colon tumor-enriched subtraction library. To evaluate this mRNA expression, surgically removed tumors as well matched normal tissue human carcinoma cell lines showing various differentiation states, anchorage dependence, proliferation states examined by Northern blot analysis. The level of (0.6 kilobase) was higher in primary than 5 6 cases. In 2 4 cases, the expression LBP (1.2 kilobases) tissue. 12 lines, poorly differentiated cells. On other hand, HLA-I (1.7 well-differentiated Although expressed both well- cells, concomitant increase with tumor progression observed two pairs derived same patients (SW480 SW620; COLO201 COLO205). Anchorage dependence butyrate-treated HT29 cells correlated lower levels mRNAs compared untreated While not changed due to growth found be low proliferating but highly induced contact-inhibited summary, therefore, high combined malignant potential.