Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules

摘要: Integration of synthetic metabolic pathways to catabolically diverse chassis provides new opportunities for sustainable production. One attractive scenario is the use abundant waste material produce a readily collectable product, which can reduce production costs. Towards that end, we established cellular platform semivolatile medium-chain α-olefins from lignin-derived molecules: constructed 1-undecene synthesis pathway in Acinetobacter baylyi ADP1 using ferulate, model compound, as sole carbon source both cell growth and product synthesis. In order overcome toxicity first applied adaptive laboratory evolution A. ADP1, resulting highly ferulate-tolerant strain. The adapted strain exhibited robust 100 mM ferulate while wild type was completely inhibited. Next, expressed two heterologous enzymes confer glucose: fatty acid decarboxylase UndA Pseudomonas putida, thioesterase ‘TesA Escherichia coli. Finally, engineered cells were able biomass solely excreted directly culture headspace. this study, employed bacterium integrate natural aromatics degrading route, allowing upgradation lignin derived molecules value-added products. We developed biosynthesis an industrially relevant chemical, 1-undecene, compound. This study reports alkenes time demonstrates potential resource bio-based valuable