Data on the standardization of a cyclohexanone-responsive expression system for Gram-negative bacteria

摘要: Engineering of robust microbial cell factories requires the use dedicated genetic tools somewhat different from those traditionally used for laboratory-adapted microorganisms. We have edited and formatted ChnR/PchnB regulatory node Acinetobacter johnsonii to ease targeted engineering ectopic gene expression in Gram-negative bacteria. The proposed compositional standard was thoroughly verified with a monomeric superfolder green fluorescent protein (msf•GFP) Escherichia coli. data presented reflect tightly controlled transcription initiation signal response cyclohexanone. Data this article are related research paper “Genetic programming catalytic Pseudomonas putida biofilms boosting biodegradation haloalkanes” [1].