Identification of a negative element in the human vimentin promoter: modulation by the human T-cell leukemia virus type I Tax protein.

摘要: The vimentin gene is a member of the intermediate filament multigene family and encodes protein expressed, in vivo, all mesenchymal derivatives and, vitro, cell types various origin. We have previously demonstrated that expression this growth-regulated could be trans activated by 40-kDa Tax HTLV-I (human T-cell leukemia virus type I) responsiveness to viral was mediated presence an NF-kappa B binding site located between -241 -210 bp upstream mRNA cap (A. Lilienbaum, M. Duc Dodon, C. Alexandre, L. Gazzolo, D. Paulin, J. Virol. 64:256-263, 1990). These previous assays, performed with deletion mutants promoter linked chloramphenicol acetyltransferase gene, also revealed negative region -529 bp. Interestingly, inhibitory activity exerted overcome after cotransfection Tax-expressing plasmid. In study, we further characterize element define effect on element. first demonstrate 187-bp domain (-424 -237 bp) behaves as when placed either or heterologous enhancer such present desmin promoter. can assigned 32-bp which indeed shown repress basal induced site.(ABSTRACT TRUNCATED AT 250 WORDS)