Mannose binding protein is involved in first-line host defence: evidence from transgenic mice.

摘要: Mannose binding protein (MBP) is a calcium-dependent C-type lectin secreted by the liver which seems to be an important component of innate or natural immunity. We have investigated effects Candida albicans and thioglycolate injection into transgenic mice bearing human MBP gene. The transgenes contained 15 kb fragment gene included complete coding sequence. Northern blot hybridization showed mRNA transcripts in two lines with low high copy number respectively. Western analysis presence serum associated higher multimeric forms are capable activating complement. Enzyme-linked immunosorbent assays (ELISA) that concentrations (1.90 +/- 0.16 mg/l, mean SEM) were about twice as levels man. concentration A, mouse homologue MBP, (13.9 0.45 mg/l) was seven times MBP. Intravenous caused level fall more than 50% first hour then slowly recover, but it did not return initial value 72 hr. 25% A rose supranormal Following increased over hr contrast remained constant both lines. These data indicate transgene include regulatory elements Total haemolytic complement activity C3 also fell immediately after while mannose specific immunoglobulin G (IgG) M (IgM) fall. plays role stages defence against infection which, this model, quantitatively greater mannose-specific IgG IgM antibodies. probably most defense previously unexposed non-immune hosts.