8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells.

摘要: 8-bromo-7-methoxychrysin (BrMC), a novel chrysin analog, was reported to have anti-cancer activities. The aim of the present study investigate molecular mechanism (BrMC)-induced apoptosis via Akt/forkhead box O3a (FOXO3a) pathway in cisplatin (DDP)-sensitive and -resistant ovarian cancer cells. human cell lines A2780 A2780/DDP were cultured vitro. Various techniques used assess expression FOXO3a B lymphoma 2 (Bcl-2)-interacting mediator death (Bim) cisplatin-sensitive Different concentrations BrMC induced BrMC-induced apoptotic occurred mainly by activation Akt, which accompanied overexpression transcription factor FOXO3a, with concomitant increase levels Bim. Silencing Bim using small interfering RNA, attenuated induction treatment. results indicated that cells may occur regulation Akt/FOXO3a, leading transcription.