Pyridoxal 5′-phosphate related changes in retention of 1,25-dihydroxy vitamin D-receptor ligands in rat intestinal mucosa cell nuclei

摘要: Abstract After feeding rats a vitamin B-6-deficient diet, we observed decrease in pyridoxal 5′-phosphate concentrations intestinal mucosa cells to 32 and 48% of control cytoplasm cell nuclei, respectively. Correlation analysis suggested that there were two pools the nuclei: “mobile” pool (equivalent about 5% concentration cytoplasmic 5′-phosphate), “stable” pool, which was independent fluctuations (about 9 pmol 5′-phosphate/mg DNA). Reduction content animals accompanied by substantial increase 1,25-dihydroxyvitamin D-receptor ligand nuclei (76.6 ± 19.7 vs 762 291 fmol/mg DNA, mean SEM). The degree 1,25-dihydrovitamin D accumulation appeared be an exponential function nuclear concentration. Semilogarithmic transformation data yielded straight line, representing inverse correlation between cytoplasm-related logarithm (r=−0.95). These suggest may related retention possibly through interaction with receptor protein nuclei.