A Sexual Dimorphism in Intrathymic Sialylation Survey Is Revealed by thetrans-Sialidase fromTrypanosoma cruzi

摘要: Sialylation is emerging as an important issue in developing thymocytes and considered among the most significant cell surface modifications, although its physiologic relevance far from being completely understood. It regulated by concerted expression of sialyl transferases along thymocyte development. After vivo administration trans-sialidase, a virulence factor American trypanosomatid Trypanosoma cruzi that directly transfers residue macromolecules, we found alteration sialylation pattern induces apoptosis inside "nurse complex." This suggests glycosylation survey development T compartment. In this study, report mechanism requires presence androgens. No increment was recorded after trans-sialidase females or antiandrogen-treated, gonadectomized, androgen receptor mutant male mice. The required only thymic epithelial cells determined bone marrow chimeric mouse approaches. CD43 mucin, molecule with still undefined function thymocytes, another absolute requirement. trans-sialidase-induced proceeds through TNF-alpha 1 deathly signaling leading to activation caspase 3. Accordingly, production cytokine increased thymocytes. ability males delete altered their reveals sexual dimorphism during compartment might be related known differences immune response sexes.