Pentatricopeptide repeat proteins in maize

摘要: Nucleus-encoded pentatricopeptide repeat proteins (PPRs) are a superfamily of ubiquitous RNA-binding factors involved in organellar gene expression. To date, large number PPR genes have been identified and some them functionally analyzed higher plants, while the family maize [Zea mays (Zm)] has long unknown. In this study, genome-scale analysis was conducted to identify all ZmPPR members, exon/intron structure, chromosomal mapping duplication events were analyzed. Then, homology modeling used explore structure-function relationships. Finally, publically transcriptome data real-time PCR for studying A total 521 PPR-encoding predicted occur genome unevenly distributed on ten chromosomes. More than 66% rarely contain introns similar structural patterns observed same classes/subclasses. Three-dimensional (3D) ZmPPR479 provided insights into mechanism RNA recognition. The transcript abundance 471 detected 60 different developmental stages reveled that members exhibit tissue-specific Under salt stress, five significantly differentially expressed found crown root (CR) primary (PR) seminal (SR), respectively. For drought expressions seven ZmPPRs showed obvious upregulation leaf, whereas downregulated. Additionally, expression 25 145 evidently increased decreased cob, Furthermore, 18 confirmed using quantitative PCR.