DISC1 Regulates Primary Cilia That Display Specific Dopamine Receptors
作者： Aaron Marley , Mark von Zastrow
DOI: 10.1371/JOURNAL.PONE.0010902
关键词:
摘要: Background Mutations in the DISC1 gene are strongly associated with major psychiatric syndromes such as schizophrenia. encodes a cytoplasmic protein many potential interaction partners, but its cellular functions remain poorly understood. We identified role of cell biology primary cilia that display disease-relevant dopamine receptors. Methodology/Principal Findings A GFP-tagged construct expressed NIH3T3 cells and rat striatal neurons localized near base cilia. RNAi-mediated knockdown endogenous resulted marked reduction number expressing cilium. FLAG-tagged versions cloned human D1, D2 D5 receptors concentrated highly on ciliary surface, this reflects specific targeting mechanism because D3 D4 to plasma membrane were not cilia. Conclusions/Significance These results identify regulating formation and/or maintenance cilia, establish subtype-specific surface. Our findings provide new insight receptor suggest relationship between neural signaling.
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