UTILIZATION OF ANTHER CULTURE FOR SCREENING OF DOUBLED HAPLOID LINES IN SOME TURKISH BREAD WHEAT HYBRIDS
作者： Ozcan YORGANCILAR , Aysel YORGANCILAR , Aysen YUMURTACU
DOI: 10.18036/BTDC.81498
关键词:
摘要: Mevcut calismada, iki Turk ekmeklik bugday cesidinin (Sonmez01 ve Es26) farkli bugdaylarla melezlenmesi sonrasi olusturulmus 30 F kokenli caprazinin katlanmis haploid (DH) verimliligini arastirmak amaclanmistir. Ozellikle verici ebeveynler yuksek verimli hastaliklara direncli yeni gelistirilmis genotiplerinden secilmistir. Tum bitki materyali 2010-2011 sezonunda Gecit Kusagi Tarimsal Arastirma Enstitusu arazisine ekilmis her gruptan gelisimin uygun evresinde ortalama 40 basak hasat edilmistir. Anter kulturu kosullari altinda, MN6 baslangic besiyeri olarak kullanilmis, 190-II rejenerasyon F2 melezlerinin ucte ikisinden kallus elde edilmis bunlar 16 albino bitkicik uretmislerdir. Kallus uretiminin genisletilmis oranlari %3.25 ile %50.5 arasinda degismistir. Yedi melez ornegi sadece %0.25 yesil uretmis, ancak bir melezde yuzdesi %6.25 hesaplanmistir. Es26’dan kokenlenmis melezlerinde hic gozlenmemisken, tum sonuclara gore, bes Sonmez01’den melezlerinden olusmustur. Ozetle, bu melezinin genom fenom temelli degerlendirilmesi, cevresel streslere karsi dayanikli secmede nesil islah platformlari icin dizayn ilave populasyon kaynagi tanimlamaya yardimci olabilir. Ayrica, calismada kullanilmis modifiye anter metodu, gelecekte diger cesitleri, hatlari tescilli genotipler populasyonu olusturulmasinda kullanilabilir.
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