Influence of Organ Environment on the Growth, Selection, and Metastasis of Human Colon Carcinoma Cells in Nude Mice

摘要: The purpose of these studies was to determine whether the biological and metastatic behaviors tumor cells isolated from fresh surgical specimens human colon carcinomas are influenced by isolation method organ site implantation growth in nude mice. Three were obtained three different patients. Two tumors primary colorectal (HCC) classified as Dukes' B2 (KM12) D stages (KM20), third a liver metastasis (KM23). enzymatically dissociated, viable implanted into subcutis or spleen mice established culture. Tumors developed both sites implantation, but hepatic metastases found only those that received splenic implantations HCC cells. Cells stage produced more disease than B tumor. parental KM12C (culture) injected cecum produce experimental spontaneous metastases, respectively. lesions harvested livers individual cell lines This procedure yielded KM12SM (spontaneous metastasis) KM12L1 (experimental metastasis). selection cycle for repeated times line designated KM12L4. selected variants routes: i.v., s.c. cecum, spleen. Subsequent spleen, all shown be tumorigenic. KM12L4 significantly higher number Moreover, subsequent injection once-selected (for incidence other lines. origin confirmed isoenzyme karyotype analyses. two highly (KM12L4 KM12SM) tetraploid elevated levels type IV collagenolytic activity. Collectively, results demonstrate orthotopic appropriate environment can used efficient study subpopulations carcinoma.