A yeast activity can substitute for the HeLa cell TATA box factor

摘要: Most class B (II) promoter regions from higher eukaryotes contain the TATA box and upstream enhancer elements1. Both elements their cognate factors have regulatory functions, whereas sequence interacts with factor BTF1 to position RNA polymerase its ancillary initiation (STF, BTF2 BTF3) direct of transcription ∼30 base pairs downstream2. In many respects, unicellular eukaryote Saccharomyces cerevisiae are similarly organized, containing activating sequences that bear similarities enhancers3,4. Although they essential for initiation, yeast located at variable distances further start sites (40–120 pairs), whose locations primarily determined by an initiator element4. The basic molecular mechanisms control known be conserved man: transcriptional trans-activator GAL4 can activate a minimal box-containing in human HeLa cells, inducible factor, oestrogen receptor, similar yeast5–8. This striking evolutionary conservation prompted us look presence activity could possibly substitute factor. We report here existence such extracts.