Immunoproteomic characterization of outer membrane vesicles from hyper-vesiculating Actinobacillus pleuropneumoniae
作者： Fabio Antenucci , Zofia Magnowska , Manfred Nimtz , Camille Roesch , Lothar Jänsch
DOI: 10.1016/J.VETMIC.2019.07.001
关键词:
摘要: Outer membrane vesicles (OMVs) are produced and secreted virtually by every known Gram-negative bacterium. Despite their non-live nature, they share antigenic characteristics with the bacteria originate from. This, together relative ease of purification, casts OMVs as a very promising flexible tool in both human veterinary vaccinology. The aim current work was to get an insight into pattern from pig pathogen Actinobacillus pleuropneumoniae context vaccine development. Accordingly, we designed protocol combining 2D Western Blotting mass spectrometric identification robustly characterize protein vesicles. Our analysis revealed that A. carry several immunoreactive virulence factors. Some these proteins, LpoA, OsmY MIDG2331_02184, have never previously been documented or other pathogenic bacteria. Additionally, showed despite abundance, proteins such FrpB DegQ do not contribute profile OMVs.
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