Immunological study of an attenuated Salmonella Typhimurium expressing ApxIA, ApxIIA, ApxIIIA and OmpA of Actinobacillus pleuropneumoniae in a mouse model.
作者： Jin HUR , Seong Kug EO , Sang-Youel PARK , Yoonyoung CHOI , John Hwa LEE
DOI: 10.1292/JVMS.14-0428
关键词: Immune system 、 Actinobacillus pleuropneumoniae 、 Virology 、 Biology 、 Microbiology 、 Antigen 、 Lymphocyte proliferation 、 Pleuropneumonia 、 Bacterial vaccine 、 Actinobacillus Infections 、 Salmonella
摘要: Salmonella Typhimurium strain expressing the Actinobacillus pleuropneumoniae antigens, ApxIA, ApxIIA, ApxIIIA and OmpA, was previously constructed as a vaccine candidate for porcine pleuropneumonia. This live attenuated (∆lon∆cpxR∆asd)Salmonella delivery host contained vector containing asd. An immunological study of lymphocyte proliferation, T-lymphocyte subsets cytokines in splenocytes mouse model carried out after stimulation with by intranasal inoculation. The splenic proliferation levels IL-4, IL-6 IL-12 inoculated mice were significantly increased, T- B-cell populations also elevated. Collectively, may efficiently induce Th1- Th2-type immune responses.
sci-hub.se 参考文章(20)
Peter J. Delves, Ivan M. Roitt, The immune system. Second of two parts. The New England Journal of Medicine. ,vol. 343, pp. 108- 117 ,(2000) , 10.1056/NEJM200007133430207
P J Mahasreshti, G L Murphy, J H Wyckoff, S Farmer, R E Hancock, A W Confer, Purification and partial characterization of the OmpA family of proteins of Pasteurella haemolytica. Infection and Immunity. ,vol. 65, pp. 211- 218 ,(1997) , 10.1128/IAI.65.1.211-218.1997
Janine T. Bossé, Håkan Janson, Brian J. Sheehan, Amanda J. Beddek, Andrew N. Rycroft, J. Simon Kroll, Paul R. Langford, Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection. Microbes and Infection. ,vol. 4, pp. 225- 235 ,(2002) , 10.1016/S1286-4579(01)01534-9
Steffi Brumme, Thorsten Arnold, Haukur Sigmarsson, Jörg Lehmann, Holger C. Scholz, Wolf-Dietrich Hardt, Andreas Hensel, Uwe Truyen, Uwe Roesler, Impact of Salmonella Typhimurium DT104 virulence factors invC and sseD on the onset, clinical course, colonization patterns and immune response of porcine salmonellosis Veterinary Microbiology. ,vol. 124, pp. 274- 285 ,(2007) , 10.1016/J.VETMIC.2007.04.032
Jan Holmgren, Cecil Czerkinsky, Mucosal immunity and vaccines Nature Medicine. ,vol. 11, ,(2005) , 10.1038/NM1213
Freddy Haesebrouck, Frank Pasmans, Koen Chiers, Dominiek Maes, Richard Ducatelle, Annemie Decostere, Efficacy of vaccines against bacterial diseases in swine: what can we expect? Veterinary Microbiology. ,vol. 100, pp. 255- 268 ,(2004) , 10.1016/J.VETMIC.2004.03.002
N RANA, R KULSHRESHTHA, Cell-mediated and humoral immune responses to a virulent plasmid-cured mutant strain of Salmonella enterica serotype gallinarum in broiler chickens. Veterinary Microbiology. ,vol. 115, pp. 156- 162 ,(2006) , 10.1016/J.VETMIC.2006.01.011
Jin HUR, John Hwa LEE, Immune responses to new vaccine candidates constructed by a live attenuated Salmonella Typhimurium delivery system expressing Escherichia coli F4, F5, F6, F41 and intimin adhesin antigens in a murine model. Journal of Veterinary Medical Science. ,vol. 73, pp. 1265- 1273 ,(2011) , 10.1292/JVMS.11-0087
Jin Hur, John Hwa Lee, Optimization of immune strategy for a construct of Salmonella-delivered ApxIA, ApxIIA, ApxIIIA and OmpA antigens of Actinobacillus pleuropneumoniae for prevention of porcine pleuropneumonia using a murine model. Veterinary Research Communications. ,vol. 38, pp. 87- 91 ,(2014) , 10.1007/S11259-013-9586-6
Ki-Weon Seo, Sae-Hae Kim, Jisang Park, Youngok Son, Han Sang Yoo, Kyung-Yeol Lee, Yong-Suk Jang, Nasal immunization with major epitope-containing ApxIIA toxin fragment induces protective immunity against challenge infection with Actinobacillus pleuropneumoniae in a murine model. Veterinary Immunology and Immunopathology. ,vol. 151, pp. 102- 112 ,(2013) , 10.1016/J.VETIMM.2012.10.011