NhaA of Escherichia coli, as a model of a pH-regulated Na+/H+antiporter.

摘要: Na(+)/H(+) antiporters are ubiquitous membrane proteins that involved in homeostasis of H(+) and Na(+) throughout the biological kingdom. Corroborating their role pH homeostasis, many antiporter regulated directly by pH. The regulation NhaA, Escherichia coli (EcNhaA), as other, both eukaryotic prokaryotic antiporters, involves a sensor conformational changes different parts protein transduce signal into change activity. Thus, residues affect response, translocation or activities cluster separate domains along molecules. Importantly, NhaA family, these conserved. Helix-packing model EcNhaA based on cross-linking data suggests, three dimensional structure response may be close proximity, forming single sensitive domain. Therefore, it is suggested that, despite considerable differences primary from bacterial to mammalian NHEs, three-dimensional architectures Test this possibility awaits atomic resolution 3D antiporters.