Cryo-EM in the Study of Membrane Transport Proteins
作者： Laura Yaunhee Kim , Matthew C. Johnson , Ingeborg Schmidt-Krey
DOI: 10.1002/CPHY.C110028
关键词:
摘要: Electron cryomicroscopy (cryo-EM) has evolved as a widely used approach to understand range of structure-function questions, particularly membrane proteins. Studies by both electron crystallography and single particle analysis have provided wealth information on transport Cryo-EM methods with an emphasis crystallography, which yielded the most protein structural any cryo-EM techniques, are described here. Two-dimensional crystallization approaches outlined, well advances in specimen preparation, data collection, image processing. Examples structure serve illustrate some understanding methods. Further examples outline impressive results that were obtained combination X-ray additional complementary
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