Lack of Fucose on Human IgG1 N-Linked Oligosaccharide Improves Binding to Human FcγRIII and Antibody-dependent Cellular Toxicity
作者： Robert L. Shields , Jadine Lai , Rodney Keck , Lori Y. O'Connell , Kyu Hong
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摘要: Lec13 cells, a variant Chinese hamster ovary cell line, were used to produce human IgG1 that deficient in fucose attached the Asn297-linked carbohydrate but otherwise similar found produced normal lines and from serum. Lack of on had no effect binding FcγRI, C1q, or neonatal Fc receptor. Although change affinity was for His131 polymorphic form FcγRIIA, slight improvement evident FcγRIIB Arg131 FcγRIIA form. In contrast, fucose-deficient FcγRIIIA improved up 50-fold. Antibody-dependent cellular cytotoxicity assays using purified peripheral blood monocytes natural killer cells several donors showed enhanced cytotoxicity, especially at lower antibody concentrations. When combined with an protein exhibited antibody-dependent lack synergistic.
sci-hub.se 参考文章(63)
D. R. Anderson, A. Grillo-López, C. Varns, K. S. Chambers, N. Hanna, TARGETED ANTI-CANCER THERAPY USING RITUXIMAB, A CHIMAERIC ANTI-CD20 ANTIBODY (IDEC-C2B8) IN THE TREATMENT OF NON-HODGKIN'S B-CELL LYMPHOMA Biochemical Society Transactions. ,vol. 25, pp. 705- 708 ,(1997) , 10.1042/BST0250705
P A Tetteroo, D Roos, A E von dem Borne, M Kerst, A Vlug, J H Nuyens, T W Huizinga, Binding characteristics of dimeric IgG subclass complexes to human neutrophils. Journal of Immunology. ,vol. 142, pp. 2359- 2364 ,(1989)
M R Clark, S B Clarkson, P A Ory, N Stollman, I M Goldstein, Molecular basis for a polymorphism involving Fc receptor II on human monocytes. Journal of Immunology. ,vol. 143, pp. 1731- 1734 ,(1989)
Pablo Umaña, Joël Jean–Mairet, Radmila Moudry, Hanspeter Amstutz, James E. Bailey, Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity Nature Biotechnology. ,vol. 17, pp. 176- 180 ,(1999) , 10.1038/6179
Robert L Shields, Angela K Namenuk, Kyu Hong, Y Gloria Meng, Julie Rae, John Briggs, Dong Xie, Jadine Lai, Andrew Stadlen, Betty Li, Judith A Fox, Leonard G Presta, None, High Resolution Mapping of the Binding Site on Human IgG1 for FcγRI, FcγRII, FcγRIII, and FcRn and Design of IgG1 Variants with Improved Binding to the FcγR Journal of Biological Chemistry. ,vol. 276, pp. 6591- 6604 ,(2001) , 10.1074/JBC.M009483200
Francoise H Routier, Michael J Davies, Klaus Bergemann, Elizabeth F Hounsell, The glycosylation pattern of humanized IgGI antibody (D1.3) expressed in CHO cells. Glycoconjugate Journal. ,vol. 14, pp. 201- 207 ,(1997) , 10.1023/A:1018589704981
Sherie L. Morrison, Ann Wright, Effect of C2-Associated Carbohydrate Structure on Ig Effector Function: Studies with Chimeric Mouse-Human IgG1 Antibodies in Glycosylation Mutants of Chinese Hamster Ovary Cells Journal of Immunology. ,vol. 160, pp. 3393- 3402 ,(1998)
M H Tao, S L Morrison, Studies of aglycosylated chimeric mouse-human IgG. Role of carbohydrate in the structure and effector functions mediated by the human IgG constant region. Journal of Immunology. ,vol. 143, pp. 2595- 2601 ,(1989)
P. A. M. Warmerdam, N. A. C. Westerdaal, A. Vlug, J. G. J. Van De Winkel, P. J. A. Capel, A single amino acid in the second Ig-like domain of the human Fc gamma receptor II is critical for human IgG2 binding. Journal of Immunology. ,vol. 147, pp. 1338- 1343 ,(1991)
B M Kumpel, P J Williams, G A Rook, T W Rademacher, I B Wilson, Galactosylation of human IgG monoclonal anti-D produced by EBV-transformed B-lymphoblastoid cell lines is dependent on culture method and affects Fc receptor-mediated functional activity. Human antibodies and hybridomas. ,vol. 5, pp. 143- 151 ,(1994)