Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter
作者： John Tebbutt , Virgil A Rhodius , Christine L Webster , Stephen JW Busby , None
DOI: 10.1111/J.1574-6968.2002.TB11159.X
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摘要: The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of RNA polymerase α subunit. We have constructed a set carrying tandem DNA sites for CRP one site centred position −61.5 and other located different upstream positions. Optimal CRP-dependent activation is observed when −93.5 or −103.5. Evidence presented to suggest that upstream-bound molecule due interaction
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