Molecular characterization and expression analysis of two farnesyl pyrophosphate synthase genes involved in rubber biosynthesis in Hevea brasiliensis

摘要: Abstract Farnesyl pyrophosphate synthase (FPS) catalyzes the formation of farnesyl from dimethylallyl pyrophosphate, which is an important precursor terpenoids, such as polyisoprenoid, in rubber-producing plants. In this paper, two genes encoding FPSs, FPS1 and FPS2, were isolated Hevea brasiliensis using PCR. The full-length cDNAs HbFPS1 HbFPS2 are 1320 1324 bp, respectively; both contain 1026-bp open reading frames that encode a polypeptide 342 amino acid residues. They share 87% 83% (HbFPS1), 78% (HbFPS2) identities with FPSs Eucommia ulmoides Parthenium argentatum, respectively. There 91% identity between HbFPS2. HbFPSs prefer T-ended codons for acids have strong asymmetric mutation bias. genomic sequences 7799 11,345 bp, respectively, comprising 12 exons 11 introns. HbFPS promoters multiple cis-regulatory elements involved responsiveness to light, hormones stress. Quantitative RT-PCR analyses showed mRNAs present all organs mature virgin rubber plants, but their transcripts most abundant reproductive organs. High expression levels individual leaf young shoots at particular positions suggested roles laticifer differentiation different phases stem bark development. significantly upregulated latex after tapping exogenous ethephon or methyl jasmonate treatments. Both severely panel dryness-affected trees downregulated healthy slightly affected bark. Thus, may play essential cis-1,4-polyisoprene synthesis trees, would provide basis further functional dissection.