Surveillance of Stem Cell Fate and Function: A System for Assessing Cell Survival and Collagen Expression In Situ

摘要: Cell-based therapy is an emerging paradigm in skeletal regenerative medicine. However, the primary means by which transplanted cells contribute to bone repair and regeneration remain controversial. To gain insight into mechanisms of how both endogenous mediate healing, we used a transgenic mouse strain expressing topaz variant green fluorescent protein under control collagen, type I, alpha 1 promoter/enhancer sequence (Col1a1GFP) membrane-bound tomato red constitutively all cell types (R26mTmG). A comparison healing parietal versus frontal calvarial defects these mice revealed that osteoblasts express Col1a1 greater degree than osteoblasts. Furthermore, scaffold-based application adipose-derived stromal (ASCs), marrow-derived mesenchymal stem (BM-MSCs), derived from critical-sized allowed for investigation su...