In vivo determination of extracellular brain ascorbate
作者： M. Miele , M. Fillenz
DOI: 10.1016/S0165-0270(96)00094-5
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摘要: A quantitative method consisting of the modification zero net flux technique was used to determine extracellular concentration ascorbate. carbon paste electrode (CPE) held at constant potential combined with a microdialysis probe through which various concentrations ascorbate were locally infused. In experiments bilateral CPEs, one dialysis probe, no difference observed in either basal or stimulated currents. Addition different perfusion fluid produced rapid changes voltammetric current due oxidation. Irrespective infused, returned level when stopped. The return found be more after infusion higher than ascorbate-free solutions. This demonstrates that homeostasis occurs vivo. calculated point 416 +/- 66 microM (n = 6). represents
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