Dopaminergic-cholinergic interactions in the striatum: the critical significance of calcium concentrations in brain microdialysis*

摘要: Brain microdialysis experiments were performed to assess the effects of calcium (1.2 mmol/l and 3.4 mmol/l) in perfusio solution on a variety pharmacological treatments known affect release dopamine (DA) and/or acetylcholine (ACh). Intrastriatal infusion muscarinic receptor agonist oxotremorine (100 μM), selective D-2 (−)-N-0437 (1 indirect DA agonists (+)amphetamine (10 μM) nomifensine via dialysis probe did not overflow ACh when perfusion fluid contained calcium. In contrast, these compounds produced pronounced decreases at 1.2 antagonist atropine increased output both mmol/1 The (−)-sulpiride either concentration. Infusion had no effect or decreased (−)-sulpirde overflow, being independent concentration fluid. Nomifensine (−)amphetamine caused relatively (but absolutely) larger increases These findings emphasize critical importance determining nature responses experiments, demonstrate that locally applied dopaminergic drugs can modulate striatal cholinergic function.