Purification of carboxyl ester lipase from human pancreas and the amino acid sequence of the n-terminal region

摘要: Summary A procedure for the purification of carboxyl ester lipase from human pancreas has been developed. The determined N-terminal 10 amino acid residues purified enzyme, NH 2 -Ala-Lys-Leu-Gly-Ala-Val-Tyr-Thr-Glu-Gly, was identical to terminal milk bile salt-activated lipase. pancreatic an apparent molecular weight slightly smaller than that (105,000 vs 125,000) as by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thus, it is possible and could be produced same gene a different splice or post-translational modification. Alternatively, they simply products two closely related but separate genes.